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Injected embryos must also be kept in the dark to avoid non-specific uncaging from ambient light.
Sub-optimal focusing can lead to unintentional uncaging of cells outside the focal plane of the target cell.
Commit securely to true wisdom the vanquishing and uncaging of craft and subtlety, which are but her two runagates.
Recent advances in imaging techniques along with increased use of two-photon glutamate uncaging have led to a wealth of new discoveries; we now suspect that there are voltage-dependent sodium, potassium, and calcium channels in the spine heads.