These tests are done using intrinsic fluorescence lifetime.
This allows for detection of counterfeit money because of the significance in difference of fluorescence lifetime when compared to authentic money.
This number varies over several orders of magnitude depending on the sample, and is known as the fluorescence lifetime of the sample.
The reading rate is limited to 10 Mbit/s because of the fluorescence lifetime.
Fluorescent proteins do not reorient on a timescale that is faster than their fluorescence lifetime.
The FRET efficiency relates to the quantum yield and the fluorescence lifetime of the donor molecule as follows:
FRET efficiency can also be determined from the change in the fluorescence lifetime of the donor.
Rotational correlation times of probe molecules in media have been measured by fluorescence lifetime or for radicals, from the linewidths of electron spin resonances.
The fluorescence lifetime refers to the average time the molecule stays in its excited state before emitting a photon.
The fluorescence lifetime is an important parameter for practical applications of fluorescence such as fluorescence resonance energy transfer and Fluorescence-lifetime imaging microscopy.