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The nature and origin of vaccine lymph, and the teachings of the new bacteriology.
He was also supplied with 'vaccine lymph' against smallpox and a medecine against cholera' (even though the cause of cholera was yet to be discovered in 1854).
It is assumed that Dr. J. Buist of Edinburgh was the first person to see virus particles in 1886, when he reported seeing "micrococci" in vaccine lymph.
He obtained and circulated in 1838 a fresh supply of vaccine lymph from cows near Berkeley, Gloucestershire, the place where Edward Jenner had originally discovered the effectiveness of vaccination in preventing smallpox.