ultrathin section

In modern electron microscopy, glass knives are used to make the ultrathin sections needed for imaging.

Ultrathin sections of the phloem tissue from suspected phytoplasma infected plants would also be examined for their presence.

After the resin has been polymerized (hardened) the sample is thin sectioned (ultrathin sections) and stained - it is then ready for viewing.

All ultrathin sections were double stained with uranyl acetate and Reynolds lead citrate and then observed under a Philips 410 microscope.

In ultrathin sections of tissues viewed by electron microscopy, the immunogold labels appear as extremely dense round spots at the position of the antigen.

Ultrathin sections for immunoelectron microscopy were washed in buffer 15 min three times and etched with saturated sodium metaperiodate for 15 min.

Semithin and ultrathin sections were cut and contrasted with lead citrate and examined with a JEOL X100 electron microscope.

This region can easily be distinguished from the "normal" membrane regions in ultrathin sections of embedded bacteria by electron microscopy when the cell membrane is orientated perpendicular to the viewing direction.

The ultrathin sections are collected on 3mm copper (mesh) grids and stained with uranyl acetate and lead citrate to make the contents of the tissue electron dense (and thus visible in the electron microscope).

Brenner's team sliced worms into thousands of ultrathin sections and photographed every section under an electron microscope, then visually matched fibers from section to section, to map out every neuron and synapse in the entire body.

Uranyl acetate and uranyl formate are used as electron-dense "stains" in transmission electron microscopy, to increase the contrast of biological specimens in ultrathin sections and in negative staining of viruses, isolated cell organelles and macromolecules.

In 1967 phytoplasmas were discovered in ultrathin sections of plant phloem tissue and named mycoplasma-like organisms (MLOs), because they physically resembled mycoplasmas The organisms were renamed phytoplasmas in 1994, at the 10th congress of the International Organization of Mycoplasmology.

Sections (1 m) were cut and stained with toluidine blue for observation by light microscopy, followed by ultrathin sections of approximately 100 nm, collected on nickel grids and stained with unranyl acetate and lead citrate, for observation on a Philips CM-10 electron microscope.

Smith has also been a primary leader in the development of array tomography (AT), a proteomic imaging method of volumetric microscopy, in which ultrathin sections of a plastic-embedded tissue are sliced using an ultramicrotome, bonded in an ordered array to a glass coverslip, stained, and imaged.