trypan blue

Lysis results in the cell taking up a dye (trypan blue).

Trypan blue is a vital stain used to selectively colour dead tissues or cells blue.

Trypan blue is so-called because it can kill trypanosomes, the parasites that cause sleeping sickness.

An analog of trypan blue, suramin is used pharmacologically against trypanosomiasis.

Trypan blue is also known as diamine blue and Niagara blue.

The percentage cell viability was checked by dye exclusion with 0.4% trypan blue under normal light microscopy.

Trypan blue is commonly used in microscopy (for cell counting) and in laboratory mice for assessment of tissue viability.

It can be measured by manual counting of cells under microscopy observation, using the dye exclusion method (i.e. trypan blue) to count only viable cells.

Trypan blue is also used in Ophtalmic cataract surgery, for staining anterior capsule, in hipermature cataracts, before capsulorhexis.

Trypan blue is derived from toluidine, that is, any of several isomeric bases, CHN, derived from toluene.

Tissues were then pinned flat on wax blocks and covered with trypan blue (0.4% solution) for 10 minutes and rinsed with PBS to improve polyp contrast.

For detection of cells general staining techniques (e.g. trypan blue) or special probes (e.g. mt-dehydrogenase detection with MTT assay) are used.

Those stains excluded by the living cells but taken up by the already dead cells are called vital stains (e.g. trypan blue or propidium iodide for eukaryotic cells).

Since cells are very selective in the compounds that pass through the membrane, in a viable cell trypan blue is not absorbed; however, it traverses the membrane in a dead cell.

In MCF7/ADR cells treated with NOM (5 μM) for 10 days the cell growth rate was normal compared with control cells, as judged by staining with trypan blue.