synoviocytes

After three passages, these primary cultures are depleted of macrophage-like type A synoviocytes [ S3 ] .

Fibroblast-like synoviocytes (FLS) represent a specialised cell type located inside joints in the synovium.

Unlike fibroblasts, fibroblast-like synoviocytes also secrete unique proteins, that are normally absent in other fibroblast lineages.

Specific for fibroblast-like synoviocytes is also the expression of CD55; this protein is often used to identify this cell type in the synovium by immunohistochemistry.

These processes are influenced by microvesicles derived from platelets, which can contribute to the activation of fibroblast-like synoviocytes through secretion of IL-1.

Retroviral transduction of primary human synoviocytes Concentrated virus was tested for its ability to transduce primary FLS.

Fibroblast-like synoviocytes that are present in the synovium during rheumatoid artritis display altered phenotype compared to the cells present in normal tissues.

The inner layer is mainly composed of two cell types, specialized macrophages (macrophage-like synovial cells) and fibroblast-like synoviocytes, which are important in maintaing the internal joint homeostasis.

The indirect ex vivo approach involves harvest of synovium, isolation and culture of synoviocytes, in vitro transduction, and injection of engineered synovicytes into the joint.

Due to the changes in proliferative and apoptotic processes the total number of cells increases in the synovium, and significantly increases also the number of fibroblast-like synoviocytes.

In the first part of the present study similar end-point levels of transgenic and recombinant IL-1Ra were necessary to inhibit fully the response of synoviocytes to a single challenge with IL-1β.

Fibroblast-like synoviocytes are cells of mesenchymal origin that display many characteristics common with fibroblasts, such as expression of several types of collagens and protein vimentin, a part of cytoskeletal filaments.

BAFF is secreted by a variety of cells: monocytes and macrophages, bone marrow stromal cells, astrocytes, synoviocytes during rheumatoid arthritis, salivary epithelial cells during Sjögren's syndrome, astrocytes in certain glioblastomas.

Contrary to the synoviocytes which are dividing cells and can be efficacy transduced in vivo using either liposomes or viral vectors, in vivo delivery of genes to chondrocytes is hindered by the dense extra cellular matrix that surrounds these cells.