southern blotting

We have discussed the inherent difficulty of Southern blotting above.

Southern blotting also led to the second breakthrough, genetic fingerprinting.

Second, Southern blotting can also be used to identify methylated sites in particular genes.

Historically, the method uses Southern blotting to find digested fragments.

A web page on Southern blotting (with his photograph)

The transformed plants were regenerated and analyzed for the presence of the gene through Southern blotting.

Southern blotting is still useful in the diagnosis of disorders caused by trinucleotide repeats.

Southern blotting and hybridisation for RARβ2 were performed by standard procedures.

This process is termed Southern blotting.

Southern blotting was used to identify positive clones which were then sequenced to provide direct evidence for the existence of the new isoform.

The ndr1-1 deletion was detected by Southern blotting done essentially as previously described [ 6 ] .

The position of the isoform sequence was then mapped to its location in the previously reported genomic clone λ1-3 (9) by Southern blotting.

The name is a dual reference to Southern blotting and the geographical concept of the "Far East" (which includes Japan).

The DNA was reamplified by a further 25 cycles and the product checked by Southern blotting.

The nested technique is often helpful, and eventually the products of polymerase chain reactions may even be analysed by Southern blotting to gain further absolute sensitivity.

For example, lysis is used in western and Southern blotting to analyze the composition of specific proteins, lipids, and nucleic acids individually or as complexes.

In November 1984 the amplification products were analyzed by Southern blotting, which clearly demonstrating increasing amount of the expected 110 bp DNA product.

Southern blotting combines transfer of electrophoresis-separated DNA fragments to a filter membrane and subsequent fragment detection by probe hybridization.

The mtDNA status of clones was determined by Southern blotting, after which Rho 0clones were maintained in medium without ethidium bromide.

These large regions were termed "ultra-long" telomeres in the literature when they were identified using southern blotting and "mega-telomeres" when identified by cytogenetic methods.

Southern blotting is an early technique basic on detection of fragments of DNA separated by size through gel electrophoresis and detected using radiolabeled probes.

The C11 and C71 loci contain at least three and two linked T-DNAs, respectively, as seen by Southern blotting (not shown).

Indeed, an IS1151-like element was located near the epsilon-toxin structural gene in all 19 type B and type D isolates studied by Southern blotting (3).

Southern blotting using the PCR product as probe was used to locate the corresponding region of the gene for genomic DNA sequencing.

A combination of Southern blotting and DNA sequencing was used to locate the position of the visceral-specific exon in the corresponding genomic clone.