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However, it was discovered afterwards that secondary interactions can be applied for improving separation power.
One of the main advantages is the very high separation power, making the technique ideal for unravelling the composition of complex mixtures.
Because of its probabilistic form, the U statistic can be generalised to a measure of a classifier's separation power for more than two classes:
IEC/HIC MMC has improved separation power and selectivity on the grounds that it applies both electrostatic and hydrophobic interactions.
The FASTpp assay combines the thermal unfolding, specificity of a thermostable protease for the unfolded fraction with the separation power of SDS-PAGE.
For example, WAX/RP has increased separation power and degree of freedom in adjusting the separation selectivity when compared with single WAX or RPLC.
Mant et al. reported that HILIC/CEX offered unique selectivity, stronger separation power and wider range of applications compared to RPLC for peptide separations.
He also said that the "wedding of all three functions of government into The Florida Bar, the 'official arm' of the Florida Supreme Court, is violative of the bedrock constitutional requirement of the separation powers and the 'checks and balances' which the separation guarantees."