selectable marker

It is used as a selectable marker in bacterial transformation.

The method is useful only for applications where selectable markers are not necessary.

The hosts are then screened with the aid of a toxic drug that the selectable marker is resistant to.

Selectable markers are used to easily differentiate transformed from untransformed cells.

A number of strategies have been developed that can remove the selectable marker from the mature transgenic plant.

An E. coli selectable marker gene, often some kind of antibiotic resistance.

In mammals, resistance to antibiotics that would kill the mitochondria is used as a selectable marker.

Using a negative selectable marker is crucial for greatly reducing the incidence of false-positives.

A selectable marker is carried by the vector to allow the selection of positively transformed cells.

A selectable marker protects the organism from a selective agent that would normally kill it or prevent its growth.

A selectable marker is a reporter gene introduced into a cell along with a gene insert.

This tends to occur at a relatively low frequency in plants and animals and generally requires the use of selectable markers.

In addition, they may allow the creation of new selectable markers for use in archaeal molecular biology.

Common to all engineered vectors are an origin of replication, a multicloning site, and a selectable marker.

Leucine synthesis then becomes a useful selectable marker.

Select plasmids for the E. coli selectable marker gene.

The construct has the mutated gene or part of it, and a reporter gene, which may be a selectable marker.

The ability to generate large numbers of hybrids using selectable markers will allow a more detailed analysis of genetic exchange in these organisms.

There are two types of marker genes: a selectable marker and a marker for screening.

These vectors generally have a selectable marker allowing the differentiation of clones containing an insert from those that do not.

It typically contains part of the gene to be targeted, a reporter gene, and a (dominant) selectable marker.

Examples of selectable markers include:

The plasmid therefore requires a selectable marker such that those cells without the plasmid may be killed or have their growth arrested.

This ensures there are no selectable markers or exogenous sequences used for targeting left in the genome that may cause unforeseen effects.

In these plasmids most of the genes have been removed, leaving only genes essential for replication and one or more selectable markers.