primer walking

Gaps in the assembled sequence may be filled by primer walking.

The term "primer walking" is used where the main aim is to sequence the genome.

Primer walking is a sequencing method of choice for sequencing DNA fragments between 1.3 and 7 kilobases.

Sequencing of 5' ends was performed on capillary sequencers (Molecular Dynamics); full-length sequencing was done on ABI377 sequencers using primer walking.

The M. pneumoniae genome sequence was published soon afterwards and was the first genome sequence determined by primer walking of a cosmid library instead of the whole-genome shotgun method.

We adopted a cDNA sequencing strategy that relies on an in vitro transposon insertion system based on the MuA transposase, combined with primer walking (see Materials and methods for details).