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FASTT is located 150 yards southwest of the primary complex.
If the Ghon focus also involves infection of adjacent lymphatics and hilar lymph nodes, it is known as the Ghon's complex or primary complex.
The contact activation pathway begins with formation of the primary complex on collagen by high-molecular-weight kininogen (HMWK), prekallikrein, and FXII (Hageman factor).
Primary inoculation tuberculosis (also known as "Cutaneous primary complex," "Primary tuberculous complex," and "Tuberculous chancre") is a skin condition that develops at the site of inoculation of tubercle bacilli into a tuberculosis-free individual.
Non-specific DNA binding still occurred, represented by the faint complex seen in lane 8 containing the pBEND2 probe with no introduced VT2 binding site, but this non-specific binding complex is a minor species compared to the specific primary complexes apparent in lanes 2-7.
Incubation mixes for lanes 2-8 contained sufficient VT2 extract to produce only primary complexes and the amount of non-specific inhibitor (polydI:polydC) was increased to 4 g per reaction to reduce the extent of non-specific VT2 binding to the non-gene 62 promoter derived probe regions.
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