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In the absence of suitable plate culture techniques, some microbes require culture within live animals.
These include a range of tubes cultures and agar plates cultures such as:
It is less clear that a pure culture comes from an animal source serving as host than it is when derived from microbes derived from plate culture.
PBMCs were separated from blood on Ficoll-Paque by differential centrifugation and were suspended in 24-well tissue culture plates culture medium.
It was a fortuitous accident: in his laboratory in the basement of St. Mary's Hospital in London (now part of Imperial College), Fleming noticed a Petri dish containing Staphylococcus plate culture he mistakenly left open, was contaminated by blue-green mould, which formed a visible growth.