peptone water

In this test, the organism under consideration is grown in peptone water broth.

The medium was replaced with fresh antibiotic free medium with D-mannose (1%) and inoculated with 25 l of an overnight culture of each E coli isolate grown in peptone water with D-mannose (1%).

Current methods of detecting and classifying cholera vibrios take several days and involve culture in alkaline peptone water (APW) and on thiosulphate/citrate/bile-salts agar, slide agglutination with specific antisera, and finally toxin assays.