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It is the space within the column that is outside of the column's internal packing material.
This creates a water-tight connection between the internal packing gaskets.
These were partially walled off at some time in the Saflieni phase; pottery shards were recovered from the internal packing of this wall.
The rotameric states of side chains and their internal packing arrangement also present difficulties in homology modeling, even in targets for which the backbone structure is relatively easy to predict.
With Jay Ponder in 1987, as part of an exploration of using internal packing of sidechains to enumerate the possible sequences compatible with a given protein backbone structure (a foreshadowing of protein engineering and design), Richards developed the first side-chain rotamer library.
