goat anti-rabbit IgG

Bound rabbit IgG was quantitated using biotinylated goat anti-rabbit IgG.

Dakopatts, Glostrup, Denmark, provided the fluorescein isothiocyanate conjugated goat anti-rabbit IgG antibody.

The samples were then incubated with alkaline phosphatase-conjugated goat anti-rabbit IgG and goat anti-mouse IgG for 30 min.

Antibodies Colloidal gold-conjugated goat anti-rabbit IgG was purchased from Ted Pella, Inc., Redding, CA.

HRP labeled goat anti-rabbit IgG was from Southern Biotechnology Associates, Inc., Birmingham, AL.

Immunoblotting was performed by using alkaline phosphatase-conjugated goat anti-rabbit IgG as a second antibody as in the Western Light TM protocol provided by Boehringer.

The secondary antibodies were Alexa 488 conjugated goat anti-rabbit IgG or anti-human IgG reagents (ALEXIS).

After three 5 min washes in PBS, sections were incubated with biotinylated goat anti-rabbit IgG antiserum for 45 min, washed in PBS, and incubated with avidin-biotin-peroxidase complex (Vector Laboratories, Burlingame, California, USA) for 45 min.