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This method is known as explant culture.
In biology, explant culture is a technique used for the isolation of cells from a piece or pieces of tissue.
In explant cultures (which allow direct cell-cell interactions) the same cells differentiate into epidermis.
The tissues used to initiate callus formation depends on plant species and which tissues are available for explant culture.
The literal meaning of tissue culture refers to the culturing of tissue pieces, i.e. explant culture.
Besides normal cultivation, D. thapsi has been propagated using explant culture, a technique employed for the isolation of cells from piece(s) of tissue for harvesting.
Antioxidant and antiinflammatory activities of ventol, a phlorotannin-rich natural agent derived from Ecklonia cava, and its effect on proteoglycan degradation in cartilage explant culture.
Explant culture can also refer to the culturing of the tissue pieces themselves, where cells are left in their surrounding extracellular matrix to more accurately mimic the in vivo environment e.g. cartilage explant culture.