clonogenic

The word "clonogenic" refers to the fact that these cells are clones of one another.

Outline the basic components of the clonogenic cell survival assay.

Attempts are now focused on developing a more rapid measure of radiosensitivity than the clonogenic assay.

Determining the basic effects of a control treatment (such as a dose-survival curve in clonogenic assay)

Perspectives on clonogenic tumor cells, stem cells, and oncogenes.

These properties can be illustrated with relative ease in vitro, using methods such as clonogenic assays, where the progeny of a single cell is characterized.

A clonogenic assay is a cell biology technique for studying the effectiveness of specific agents on the survival and proliferation of cells.

Growth of fibroblasts as a potential confounding factor in soft agar clonogenic assays for tumour cell radiosensitivity.

An ex vivo assay for examining the clonogenic potential of multipotent marrow cells was later reported in the 1970s by Friedenstein and colleagues.

He developed the first in vivo clonogenic assay for hepatocytes, and used it to quantify their survival when exposed to X-rays and neutrons.

MDM2 depletion in the absence of p53 promoted the differentiation of human mesenchymal stem cells and diminished clonogenic survival of cancer cells.

In 1955 he co-authored a paper on "the first practical and efficient method for growing colonies from individual animal cells", known as the clonogenic assay, which is still used today.

Over fifty years later he documented that a luncheon conversation with Leó Szilárd, the nuclear physicist, led to the concept and physical setup for the first clonogenic assay.

Properties of stem cells can be illustrated in vitro, using methods such as clonogenic assays, in which single cells are assessed for their ability to differentiate and self-renew.

In Colorado, he became codiscoverer of the clonogenic assay and the feeder cell system, making it possible to grow clones from single mammalian cells as in stem cells.

Enhanced levels and enhanced clonogenic capacity of blood progenitor cells following administration of SCF plus G-CSF to humans.

Cytotoxicity can also be measured by the sulforhodamine B (SRB) assay, WST assay and clonogenic assay.

Similarly, when cells are irradiated, and the medium is transferred to unirradiated cells, these unirradiated cells show bystander responses when assayed for clonogenic survival and oncogenic transformation.

Moreover, this adhesive interaction resulted in the growth inhibition of both clonogenic erythroid (BFU-E) and myeloid (CFU-GM) progenitors by more than 90 per cent.

Our study involves a specific, well-characterized population of undifferentiated cells: the c-kit-positive cardiac stem cells are self-renewing, clonogenic and multipotent, which are the fundamental properties of stem cells."

Cell viability assays: resazurin method, ATP test, Ethidium homodimer assay (detect dead or dying cells), Bacteriological water analysis, Clonogenic assays, ...

Similarly, GRN163L inhibited the in vitro clonogenic growth of CD138neg Multiple Myeloma Cancer Stem Cells isolated from the bone marrow aspirates of patients with multiple myeloma.

Further studies reported that [Nle,-Phe]α-MSH did not enhance anchorage-independent clonogenic cell growth, a hallmark of malignancy, and it had no effect on tumor incidence, size or on metastatic spread in in vitro and animal models.

Additional testing Any assays performed on HPC (e.g. nucleated cells, mononuclear cells, CD 34 positive cells and/or clonogenic assays) should be performed in a nationally accredited laboratory which participates in appropriate external quality assessment schemes.

Equivalent Uniform Dose (EUD) is one such model, and was introduced by Niemierko [1] as a means of relating a 3D non-uniform physical dose delivered to clonogenic cells, to a single dose value which has an equivalent radiobiological effect.