bromophenol blue

Bromophenol Blue solution can be used for the same purpose.

Bromophenol blue and orange G can also be used for this purpose.

Bromophenol blue is also used as a dye.

Bromophenol blue is structurally related to phenolphthalein (a popular indicator).

Gel was run at 15 mAmps constant current until the leading dye band (bromophenol blue) migrated near the bottom.

Examples of dichromatic substances are pumpkin seed oil, bromophenol blue and resazurin.

Bromophenol blue is the substance with the highest known value of Kreft's dichromaticity index.

Bromophenol blue is commonly used in entry-level lab courses to stain proteins in wet-mount slides.

Immediately before loading, 2 l of loading buffer (binding buffer containing 50% glycerol and 0.1 mg/ml bromophenol blue) were added to the samples.

Bromophenol blue is also used as a color marker to monitor the process of agarose gel electrophoresis and polyacrylamide gel electrophoresis.

A very common tracking dye is Bromophenol blue (BPB, 3',3",5',5" tetrabromophenolsulfonphthalein).

Commonly used color markers include Bromophenol blue, Cresol Red, Orange G and Xylene cyanol.

Following incubation of the mixture for 10 min at 4 C, equal volume of 2x gel-loading dye containing 0.25% bromophenol blue and 20% glycerol was added.

The digestion reaction was stopped after 12 seconds by addition of 4 l formamide containing 10mM EDTA and 0.1% (w/v) bromophenol blue.

Bromophenol blue (3',3",5',5"-tetrabromophenolsulfonphthalein, BPB, albutest) is used as an acid-base indicator, a color marker and a dye.

For electrophoresis, 20 μg of RNA per sample was resolved on a 1% agarose gel containing 2.2 M formaldehyde at 5V/cm until the bromophenol blue migrated 8 cm.

Reactions were stopped by addition of loading buffer: 80% formamide, 10 mM Na 2 EDTA (pH 8.0), and 1 mg/ml each bromophenol blue and xylene cyanol.

Reactions were terminated by the addition of an equal volume of loading dye (8 M urea, 10% sucrose, 40 mM EDTA, 0.1% bromophenol blue, 0.1% xylene cyanol).

After incubation at 37 C for up to 20 min, an equal volume of 90% formamide, 10 mM KOH, 0.25% bromophenol blue and 0.25% xylene cyanol was added to stop the reactions.

Commonly used are the dye pairs xylene cyanol and bromophenol blue; these migrate at approximately the same rate as DNA fragments 4000 and 500 base pairs in length respectively in a 1% agarose gel.

It was stopped after one minute with 25 l of 9% SDS sample buffer (0.0015% bromophenol blue, 10 ml of 2% SDS, 25 mM TRIS pH 6.8, and 20% sucrose).

Generally speaking, Orange G migrates faster than bromophenol blue, which migrates faster than xylene cyanol, but the apparent "sizes" of these dyes (compared to DNA molecules) varies with the concentration of agarose and the buffer system used.

At this time a 4.5 μl aliquot was removed for a zero time point and quenched with 5 μl stop dye (95% formamide, 20 mM EDTA, 0.5% xylene cyanol, and 0.5% bromophenol blue).

The DNA was then extracted by phenol, chloroform and ethanol precipitation and resuspended in formamide dye mix (1mg/ml xylene cyanol, 1mg/ml bromophenol blue and 10mM EDTA in deionized formamide).

Xylene cyanol and Bromophenol blue are common dyes found in loading buffers; they run about the same speed as DNA fragments that are 5000 bp and 300 bp in length respectively, but the precise position varies with percentage of the gel.