autofluorescent

Intracellular deposition of autofluorescent material-persumably lipofuschin occurs throughout the body.

In contrast, autofluorescent background consists of a multitude of individual emitters, which obey individual emission statistics.

Neurons labeled by PRV-GFP were detectable because they were autofluorescent.

In both studies, spectral imaging and autofluorescent subtraction allowed multicolour in vivo visualization of cells and tissues.

In marine biology, the autofluorescent properties of photosynthetic plankton can be exploited by flow cytometry in order to characterise abundance and community structure.

The Super resolution microscopy SPDM revealed autofluorescent cellular objects which are not detectable under conventional fluorescence imaging conditions.

The main application of FIDSAM is the discrimination of unspecific autofluorescent background signal from the target signal of a dedicated chromophore.

Beside a substantial resolution improvement of autofluorescent structures, using two different laser wavelengths SPDM reveals cellular objects which are not detectable under conventional fluorescence wide-field imaging conditions.

Activated or ameboid microglia and macrophages that contain myelin debris, lipid droplets and brown autofluorescent pigment granules are found in the areas with demyelination and axonal spheroids.

These studies took advantage of the fact that NAPDH, in its reduced form, is autofluorescent, and that metabolites such as glucose cause a predictable increase in NAPDH reduction.

Recently, structured illumination light microscopy, using a specially designed super resolution microscope setup has been used to resolve the fluorescent distribution of small autofluorescent structures (lipofuscin granulae) in retinal pigment epithelium tissue sections.

This mechanical separation of autofluorescent red blood cells allows for the imaging and counting of white blood cells from unprocessed whole blood without additional sample processing, such as centrifugation or red blood cell lysis.

GFP and its variants are autofluorescent proteins that can be visualized in living cells, and are therefore particularly useful for monitoring gene expression in whole embryos, animals or cultured cells and organs [ 15].

Detection and relational mapping of tagged loci configuration As plants contain chloroplasts in leaf and stem cells and many autofluorescent compounds are localized in cell walls, a signal-to-background ratio for GFP detection is probably lower than in animal systems.