acridine orange

The ring structure of the acridine orange can absorb the incoming radiation.

Acridine orange is prepared from coal tar and creosote oil.

Acridine orange binding with the nucleic acid occurs in both living and dead bacteria, also other microorganisms.

Since then the use of acridine orange has been performed frequently in the examination of soil and water for microbial content.

Acridine yellow is similar to acridine orange.

Thus, acridine orange can be used to identify engulfed apoptotic cells, because it will fluoresce upon engulfment.

All slides were examined under 1000x magnification using a fluorescent microscope fitted with appropriate filters for acridine orange stain.

Acridine orange (3,6-dimethylaminoacridine) is a nucleic acid-selective metachromatic stain useful for cell cycle determination.

The stain may also be used in conjunction with acridine orange (AO) in viable cell counting.

Acridine orange will also enter acidic compartments such as lysosomes and become protonated and sequestered.

Acridine orange can be used in conjunction with ethidium bromide to differentiate between viable, apoptotic and necrotic cells.

It is referring to the hydrophobic nature of the compound; acridine orange tends to diffuse spontaneously into the membrane surrounding the microorganisms.

In 1942, Hilbrich and Strugger were first described using acridine orange to detect the fluorchromatic staining of microorganisms.

Acridine derivatives: proflavin, acridine orange, acridine yellow etc.

Affected cells do not exhibit hallmarks of apoptosis but rather autophagy as seen by staining with acridine orange and immunoreactivity for LC3.

Psoralens, coal tars, photo-active dyes (eosin, acridine orange)

The use of acridine orange in clinical applications has become widely accepted; mainly focusing on the use in highlighting bacteria in blood cultures.

Direct epifluoresent filter technique (DEFT) using acridine orange is specified in methods for the microbial examination of food and water3.

They applied high-voltage alternating current (AC) fields in air to materials such as acridine orange, either deposited on or dissolved in cellulose or cellophane thin films.

Acridine orange can be used to differentiate between deoxyribonucleic acid (DNA) and ribonucleic acid (RNA) very easily.

In organic compounds, electroluminescence has been known since the early 1950s, when Bernanose and coworkers first produced electroluminescence in crystalline thin films of acridine orange and quinacrine.

The results showed that the acridine orange is a simple, inexpensive, rapid staining procedure that appeared to be more sensitive than the Gram stain for detecting microorganism in clinical materials1.

Acridine orange is a versatile fluorescence dye to stain acidic vacuoles (lysosomes, endosomes, and autophagosomes), RNA, DNA in living cells.

Acridine orange emits yellow fluorescence when it binds RNA and green fluorescence when it binds DNA.