Ti plasmid

This detection is the first stage in the Ti plasmid transfer.

The Ti plasmids are classified into different types based on the type of opine produced by their genes.

At least 25 vir genes on the Ti plasmid are necessary for tumor induction.

Ti plasmids are classified on the basis of the different types of opines they produce.

The Agrobacterium tumefaciens infects the plant through its Ti plasmid.

Many plant expression vectors are based on the Ti plasmid of Agrobacterium tumefaciens.

They developed a vector system for transferring foreign genes into the plant genome, by using the Ti plasmid of Agrobacterium tumefaciens.

She and her collaborators produced the first genetically modified plants using Agrobacterium carrying the disarmed Ti plasmid (1983).

They are artificial vectors that have both been created from the naturally occurring Ti plasmid found in Agrobacterium tumefaciens.

Agrobacterium tumefaciens traR (regulation of Ti plasmid transfer)

Tumour inducing plasmid (Ti plasmid):

Ti plasmid vector for introduction of DNA into plant cells without alteration of their normal regeneration capacity.

Transfer is initiated at the right border and terminated at the left border and requires the vir genes of the Ti plasmid.

Since the Ti plasmid is essential to cause disease, pre-penetration events in the rhizosphere occur to promote bacterial conjugation - exchange of plasmids amongst bacteria.

The virG protein is a cytoplasmic protein produced from the virG Ti plasmid gene.

The first step in integrating the T-DNA into a host genome is the formation of nick at the right border of the Ti plasmid.

The cryptic plasmid of 'Agrobacterium tumefaciens' cointegrates with the Ti plasmid and cooperates for opine degradation.

It was discovered in Agrobacterium tumefaciens, which uses this system to introduce the Ti plasmid and proteins into the host, which develops the crown gall (tumor).

Agrobacterium naturally inserts DNA into plants from its Ti plasmid, and scientists use this to insert genes of interest into various plants.

The use of the gene gun may be contrasted with the use of Agrobacterium tumefaciens and its Ti plasmid to insert genetic information into plant cells.

The former is recognised by the VirA protein, a transmembrane protein encoded in the virA gene on the Ti plasmid.

Two nopaline type Ti plasmids, pTi-SAKURA and pTiC58, were fully sequenced.

The Ti plasmid is lost when Agrobacterium is grown above 28 C. Such cured bacteria do not induce crown galls, i.e. they become avirulent.

The Ti plasmid also contains the genes for opine catabolism produced by the crown gall cells, and regions for conjugative transfer and for its own integrity and stability.

She was the first (1977) to demonstrate the presence of a fragment of Agrobacterium Ti plasmid DNA in the nuclear DNA of crown gall tissue.