suspension culture
cell suspension

Many types of systems have been developed for patch clamping cells in suspension cultures.

Where it is important to retain some degree of normal morphogenesis, fractions should be grown in suspension culture.

Friable calluses fall apart easily, and can be used to generate cell suspension cultures.

Since neurons don't typically grow in suspension cultures, however, other cell types are typically used.

Tobacco BY-2 cells (kept as cell suspension culture, they are model system of plant cell)

The drawback to suspension culture patch clamping is that the cells or ion channels are typically not in their natural environment.

Moreover, plastic beads of polystyrene, sephadex and polyacrylamide are also available for cell growth in suspension culture.

Suspension cultures are easily passaged with a small amount of culture containing a few cells diluted in a larger volume of fresh media.

One system uses a traditional pipette and cells in a droplet suspension culture to obtain patch clamp recordings (see figure).

In cell suspension cultures, each of the cells is floating independently or at most only in short chains in a culture medium.

In parallel, MDAH cells were also treated with tetracycline and transferred to suspension culture.

Because the cells are dissociated from one another in a suspension culture, the ionic currents in that single cell can be measured in detail.

A photobioreactor can be described as an enclosed, illuminated culture vessel designed for controlled biomass production of phototrophic liquid cell suspension cultures.

Furthermore, increased MMP expression coincided with decreased ability of P19 cells to aggregate in suspension culture.

Following cell thaw, NSs were expanded in suspension culture, and in each case were used between passages 5 and 7.

The moss bioreactor is used to cultivate moss in a suspension culture in agitated, and aerated liquid medium.

The first documentation of somatic embryogenesis was by Steward et al. in 1958 and Reinert in 1959 with carrot cell suspension cultures.

IDO expression was detected when P19 cells were reseeded into bacterial petri dishes as suspension cultures and allowed to form aggregates.

At 30 hours after seeding suspension cultures, cells formed irregular shaped, non-spherical aggregates that were less tightly packed than control aggregates.

MFP1 contains an extended coiled-coil domain and is located at the nuclear periphery of tobacco suspension culture cells [ 24 25 ] .

After the induction of p53 was maximal, the cells were transferred to suspension culture conditions and analyzed for their expression of PTEN.

It should be noted that the fibroblast cells lines we used remain fully viable after detachment and transfer to suspension culture [ 31 43 ] .

In the 1990s large-scale production of the virus in cell suspension cultures of Drosophila or T.ni cells made x-ray crystallographic studies feasible.

We next analyzed whether the elevated quantity of PTEN protein would indeed be reflected in increased phosphatase activity in suspension culture cells.

As shown in Figure 1, this transfer to suspension culture resulted in elevated expression of PTEN protein in each cell line.