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The cells were grown in antibiotic free medium for two days to give a semiconfluent monolayer of cells on each coverslip.
People with semi-confluent and confluent types of smallpox may have therapeutic issues similar to patients with extensive skin burns.
Total RNA was isolated from semi-confluent cell cultures using the TRIzol reagent (Life Technologies, Gaithersburg, MD) according to the manufacturer's protocol.
A semi-confluent element need not be confluent, but a semi-confluent rewriting system is necessarily confluent, and a confluent system is trivially semi-confluent.
