plasmid DNA

Many methods have been developed to purify plasmid DNA from bacteria.

This method works very well for circular plasmid DNA.

In a process called transformation, plasmid DNA is taken up by the bacteria under a variety of conditions.

Plasmid DNA was isolated from transformants and screened by size.

Each transfection was repeated at least four times with two different plasmid DNA preparations.

Plasmid DNA itself appears to have an adjuvant effect on the immune system.

However, it requires plasmid DNA of high quality and it is time-consuming.

Standard procedures were used for plasmid DNA manipulations (13).

This results in relatively large amounts (several micrograms) of very pure plasmid DNA.

Plasmid DNA was isolated from the clones and the sequences were verified.

Plasmid DNA was isolated and sequenced in order to verify the sequence identity.

Plasmid DNA integrity was confirmed by restriction analysis and cycle sequencing.

Comparison of sequencing with plasmid DNA templates generated by three different methods.

Otherwise, smearing may occur in enzyme restricted form of plasmid DNA.

Plasmid DNA of 8-10 different positive recombinant clones was isolated and sequenced.

From his microbial mix, he hopes to find the plasmid DNA responsible for the degradative ability.

Sequencing was performed on plasmid DNA by the method of Sanger (35).

The predicted 5' and 3' boundaries of the expression regions were confirmed by direct plasmid DNA sequencing.

- A continuous thermal lysis procedure for the large-scale preparation of plasmid DNA.

The payload is an elemental particle of a heavy metal coated with plasmid DNA.

After a good number of bacterial colonies have grown, they can be miniprepped to harvest the plasmid DNA.

Plasmid DNA was harvested from the transformants, ethanol precipitated, and resuspended in water.

Introduction of plasmid DNA into the mouse airways was as described in the legend to Fig. 2.

With the samples looking good, the authors turned to sequencing the Y. pestis plasmid DNA.

It increases the ability of a prokaryotic cell to incorporate plasmid DNA allowing them to be genetically transformed.