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It is not yet known if gene probes can outfox this viral trick.
Now added to these screening techniques are so-called gene probes or genetic marker tests.
Each person would donate a blood sample and researchers would search their chromosomes with "gene probes," genetic markers whose location is known.
If the smear is positive, PCR or gene probe tests can distinguish M. tuberculosis from other mycobacteria.
Test for Huntington's Disease One of the tests is a gene probe for Huntington's disease, a degenerative brain ailment that strikes around age 40.
Currently, DNA microarrays are manufactured using either cDNA or oligonucleotides as gene probes.
Complementary DNA is often used in gene cloning or as gene probes or in the creation of a cDNA library.
In these experiments [ 20 21 22 23 ] , regardless of the gene selection method used, less than 5% of the total number of genes probed were differentially regulated.
The figure above represents the output of a two dimensional cluster, in which similar samples (rows, above) and similar gene probes (columns) were organized so that they would lie close together.
If the results of a gene probe (rpoB) are known to be positive, then it is reasonable to omit RMP and to use SHEZ+MXF+cycloserine.
From the UniGene1 EST assembly 5,376 cDNA gene probes were used to fabricate a spotted cDNA microarray for a suite of hybridizations.
We stress that these techniques are meaningful only when the number of genes probed is sufficiently large, and under the assumption that a large fraction of them does not show differential regulation between the two tested samples.
Although it is widely acknowledged that DNA arrays are most powerful when they contain as many different gene probes as possible, the cost of purchasing or producing such large arrays is prohibitive for most researchers.
Next, she'd denatured the DNA with alkali and transferred the fragments to a nitrocellulose filter, which she'd then exposed to a specific radio-actively labelled gene probe she'd designed in Chicago and brought over with her.
A gene probe for rpoB is available in some countries, which serves as a useful marker for MDR-TB, because isolated RMP resistance is rare (except when patients have a history of being treated with rifampicin alone).
The observation that cells reacted with the ATPase 6 gene probe are located in these two areas suggests that type II pneumocytes and Clara cells are the cell types that over-expressed the ATPase 6 gene.
The purpose of this chapter is to describe some of the available techniques and the ways of utilizing the preparations obtained by them in a variety of applications which range from the simple determination of chromosome counts to the in situ hybridization of gene probes to banded chromosomes.