DNA topoisomerase

More information about this protein can be found at Protein of the Month: DNA Topoisomerase.

DNA topoisomerase 1 is an enzyme that in humans is encoded by the TOP1 gene.

Savene works by inhibiting DNA topoisomerase II, which is the target of anthracycline chemotherapy.

Pommier Y. DNA topoisomerase I inhibitors: chemistry, biology, and interfacial inhibition.

As DNA topoisomerase inhibitors, fisetin and other flavonoids may increase risk of infant acute myeloid leukemia, a rare disease.

Inhibition of DNA topoisomerase II also plays an important role in the cytotoxic activity of genistein.

This hypothesis could be tested using other DNA topoisomerase poisons known to induce NF-κB [ 34].

Human DNA topoisomerase I (Top1) is an essential enzyme that relaxes DNA supercoiling during replication and transcription.

The transesterification domains of these eukaryotic enzymes have high degrees of identity to E. coli DNA topoisomerase III [ 7 37 ] .

The doxorubicin damages DNA by intercalation, the generation of reactive oxygen species and inhibition of DNA topoisomerase I and II.

The general modes of action found in mammalian cells are inhibition of protein kinases, inhibition of eukaryotic DNA topoisomerase, and intercalative binding to DNA.

The integrity of the DNA was maintained by a group of maintenance enzymes, including DNA topoisomerase, DNA ligase and other DNA repair enzymes.

The drug binds to DNA topoisomerase II at a different step in the catalytic cycle than anthracyclines, which locks the enzyme in a conformational form that is no longer affected by anthracyclines.

Reversible association between pradofloxacin and DNA gyrase or DNA topoisomerase IV in the target bacteria results in inhibition of these enzymes and rapid death of the bacterial cell.

The technique utilises the inherent biological activity of DNA topoisomerase I. The biological role of topoisomerase is to cleave and rejoin supercoiled DNA ends to facilitate replication.

Conclusions We have shown that the ZD domain of E. coli DNA topoisomerase I is not required for the substrate recognition and DNA cleavage-religation action of the enzyme.

Materials and methods Enzyme and DNA E. coli DNA topoisomerase I and the ZD domain were expressed and purified as described [ 6 41 ] .

Conversely, the enzyme DNA topoisomerase I is nonessential in S. cerevisiae and S. pombe, but essential for viability in the pathogenic fungus Cryptococcus neoformans [ 24 ] .

Background Escherichia coli DNA topoisomerase I is a representative example of type IA DNA topoisomerase (for reviews, see refs [ 1 2 ] ).

Yun J, Tomida A, Nagata K, Tsuruo T. Glucose-regulated stresses confer resistance to VP-16 in human cancer cells through a decreased expression of DNA topoisomerase II.

Two clinical subgroups of patients have a high frequency of AML with 11q23 abnormalities: AML in infants and therapy-related AML, usually occurring after treatment with DNA topoisomerase inhibitors.

Mitoxantrone is an immunosuppressant drug and it prevents the proliferation of T-cells by inhibiting DNA repair proteins and DNA topoisomerase, which are vital in the DNA replication process before mitosis.

Background DNA topoisomerase I (TOP1) is a ubiquitously expressed protein that relaxes DNA supercoils generated during many transactions of DNA including replication, transcription, repair, and chromatin condensation and remodeling.

In E.coli, DNA topoisomerase IV plays the major role in the separation of the catenated chromosomes, transiently breaking both DNA strands of one chromosome and allowing the other chromosome to pass through the break.

HL-60 cell model was used to study the effect of DNA topoisomerase (topo) IIα and IIβ on differentiation and apoptosis of cells and is especially useful in dielectrophoresis studies, which require an aqueous environment with suspended and round cells.