reverse transcription polymerase chain reaction

A direct confirmation can be obtained by reverse transcription polymerase chain reaction where the genome of the virus is amplified.

Absolute quantification of mRNA using real-time reverse transcription polymerase chain reaction assays.

Despite the fact that the reverse transcription polymerase chain reaction has been widely used in research laboratories, its clinical utility in chronic hepatitis C is not established.

Malaria-infected individuals may harbour gametocytes below the microscopic detection threshold that can be detected by reverse transcription polymerase chain reaction targeting gametocyte-specific mRNA.

Another detection assay is the reverse transcription polymerase chain reaction (RT-PCR) tests which may detect nucleic acids in the blood and cerebrospinal fluid.

The exponential amplification via reverse transcription polymerase chain reaction provides for a highly sensitive technique in which a very low copy number of RNA molecules can be detected.

The expression of Tumor necrosis factor-alpha mRNA was measured at 3,8, and 24 hours after CLP using reverse transcription polymerase chain reaction.

Single cell sorting and reverse transcription polymerase chain reaction The method for construction of cDNA libraries from single cells was similar to previously reported techniques [ 21, 22].

Reverse transcription polymerase chain reaction (RT-PCR) is one of many variants of polymerase chain reaction (PCR).

Reverse Transcription Polymerase Chain Reaction (RT-PCR) is a variation of PCR that can be used to quantify viral RNA.

Chromosome banding analysis, fluorescence In Situ hybridization (FISH), and reverse transcription polymerase chain reaction (RT-PCR) are common methods employed at diagnostic laboratories.

Reverse transcriptase is commonly used in research to apply the polymerase chain reaction technique to RNA in a technique called reverse transcription polymerase chain reaction (RT-PCR).

In most cases, cell culturization is recommended for surveillance, with antibody tests and reverse transcription polymerase chain reaction (RT-PCR) and genetic sequencing and comparison for definitive confirmation and genotype classification.

Laboratory tests such as reverse transcription polymerase chain reaction (RT-PCR), Western blotting, negative-staining immunoelectron microscopy and ELISAs may be performed on samples from the liver, blood, spleen or other organs.

Routine protocols to detect norovirus (norovirus RNA) in clams and oysters by reverse transcription polymerase chain reaction are being employed by governmental laboratories such as the FDA in the USA.

Reverse transcription polymerase chain reaction (RT-PCR) Following experimental treatments, cells were harvested and total RNA was isolated according to manufacturer's instructions (Tri Reagent, Molecular Research Center, Cincinnati, OH).

However, the expression of this snoRNA could not be confirmed by northern blotting or Reverse transcription polymerase chain reaction (RT-PCR) and it should not be confused with this snoRNA located in the nucleolin gene.

A four-layer glass-PDMS device has been developed using this architecture integrating microvalves, microheaters, temperature sensors, 380-nL reaction chambers, and capillary electrophoresis channels for reverse transcription polymerase chain reaction (RT-PCR) that has attomolar detection sensitivity.

To evaluate the relative expression of Tnfrh1 and Tnfrh2 in various organs and tissues, we performed reverse transcription polymerase chain reaction (RT-PCR) in multiplex reactions using a shared downstream primer and Tnfrh1 - and Tnfrh2 -specific upstream primers.

Assays for the laboratory diagnosis of SARS-CoV infection include enzyme-linked immunosorbent assay, indirect fluorescent-antibody assay, and reverse transcription polymerase chain reaction (RT-PCR) assays of appropriately collected clinical specimens (Source: CDC.

Detection of the nucleic acid of BAV can be performed with a TaqMan reverse transcription polymerase chain reaction (RT-PCR) assay by extracting the RNA from a sample and reverse transcribing it into detectable cDNA.

Students and scientist alike often confuse this method with the technique called "Reverse transcription polymerase chain reaction" (RT-PCR), which includes the reverse transcription of RNA to DNA but does not necessarily include the quantification of the product in real time.